Hi, To purify your protein you can use GST-Trap HP column, it is quite easy, you have to elute in a buffer with 20mM reduced glutathione.
HTH, Catarina -- Catarina Rodrigues, PhD Student Molecular Transport & Signalling AFMB UMR 6098 CNRS/UI/UII Case 932 163 Avenue de Luminy 13288 Marseille cedex 9 (France) Tel : +33 04 91 82 55 60 Fax : 04 91 26 67 20 e-mail:catarina.rodrig...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/ Le 27 mai 2011 à 22:50, Pascal Egea a écrit : > > Dear All, > > This is not strictly a crystallography related question. > We are trying to express several membrane proteins and were considering the > use of GST as a fusion partner instead of the HIS or FLAG purification tags. > I would like to know if anyone had any experience (positive or negative) to > share with us. > > Many thanks in advance. > > -- > Pascal F. Egea, PhD > Assistant Professor > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > 364 Boyer Hall > Molecular Biology Institute > office (310)-983-3515 > lab (310)-983-3516 > email pe...@mednet.ucla.edu > > > -- > Pascal F. Egea, PhD > Assistant Professor > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > 364 Boyer Hall > Molecular Biology Institute > office (310)-983-3515 > lab (310)-983-3516 > email pe...@mednet.ucla.edu > > > -- > This message has been scanned for viruses and > dangerous content by MailScanner, and is > believed to be clean. -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
