Hi Pascal, The GST helps a lot with expression, but unfortunately it's very difficult to purify it away after cleavage. I would strongly recommended to try MBP tag.
Good luck! Anna On Fri, May 27, 2011 at 1:50 PM, Pascal Egea <pas...@msg.ucsf.edu> wrote: > > Dear All, > > This is not strictly a crystallography related question. > We are trying to express several membrane proteins and were considering the > use of GST as a fusion partner instead of the HIS or FLAG purification tags. > I would like to know if anyone had any experience (positive or negative) to > share with us. > > Many thanks in advance. > > -- > Pascal F. Egea, PhD > Assistant Professor > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > 364 Boyer Hall > Molecular Biology Institute > office (310)-983-3515 > lab (310)-983-3516 > email pe...@mednet.ucla.edu > > > -- > Pascal F. Egea, PhD > Assistant Professor > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > 364 Boyer Hall > Molecular Biology Institute > office (310)-983-3515 > lab (310)-983-3516 > email pe...@mednet.ucla.edu > >
