Dear All, This is not strictly a crystallography related question. We are trying to express several membrane proteins and were considering the use of GST as a fusion partner instead of the HIS or FLAG purification tags. I would like to know if anyone had any experience (positive or negative) to share with us.
Many thanks in advance. -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry 364 Boyer Hall Molecular Biology Institute office (310)-983-3515 lab (310)-983-3516 email pe...@mednet.ucla.edu -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry 364 Boyer Hall Molecular Biology Institute office (310)-983-3515 lab (310)-983-3516 email pe...@mednet.ucla.edu
