James Holton wrote:
In general, a Bijvoet ratio of 3% or so is needed to solve a structure (the
current world record is 0.5% and lots of multiplicity). The above web page
will also tell you how many crystals you need if you type in their size in all
three dimensions. but this estimate assum
Jacob,
The main reason why it is not common practice to saturate every crystal
with every heavy metal under the sun is radiation damage. X-ray
absorption increases very rapidly with atomic number (third power), so
on the order of 100 mM of "heavy atom" is usually enough to cut your
crystal'
iodide conc. for SAD data
To: CCP4BB@JISCMAIL.AC.UK
Yes I did and could solve the structure by SAD.
In total 8 I atoms found. 6 bound at anion binding sites. 2 as I2.
None covalently bound to tyrosine.
Florian
Am 03.05.12 19:42, schrieb
Yes I did and could solve the structure by SAD.
In total 8 I atoms found. 6 bound at anion binding sites. 2 as I2.
None covalently bound to tyrosine.
Florian
Am 03.05.12 19:42, schrieb Edward A. Berry:
> Did you locate the Iodine(s)? Did you have iodotyrosine, or I^- bound
> at anion binding si
Did you locate the Iodine(s)? Did you have iodotyrosine, or I^- bound
at anion binding sites? There are two distinct methods based on I2/I-.
Florian Sauer wrote:
Dear Rajesh,
another method to incorporate Iodine into your crystal is by simply
placing a drop of KI/I2 solution next to the cryst
Dear Rajesh,
another method to incorporate Iodine into your crystal is by simply
placing a drop of KI/I2 solution next to the crystallization drop.
Have a look here:
Acta Cryst. (2006). D62, 280-289
New methods to prepare iodinated derivatives by vaporizing iodine
labelling (VIL) and hydrogen pe
[CCP4BB@JISCMAIL.AC.UK] on behalf of Roger Rowlett
[rrowl...@colgate.edu]
Sent: Thursday, May 03, 2012 11:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] effective iodide conc. for SAD data
Interesting idea. The only caveat that springs to mind is that the more useful
anions (e.g., iodide and brom
Interesting idea. The only caveat that springs to mind is that the more
useful anions (e.g., iodide and bromide) are on the chaotropic end of
the Hofmeister series and may potentially destabilize protein structure
or protein-protein interactions, which might complicate
co-crystallization starti
I have wondered for a long time now why it is not standard practice for all
crystallization protein stocks to contain either Br- or I- ions instead of
Cl-, even for cationic buffers like TRIS, which could be titrated with HBr
or HI to get in the 10+ mM range. Also, one could use Cs or Rb for the
ca
Rajesh,
Why not try a soak-in-place by adding iodide and/or cryoprotectant
directly to the crystallization drop? Then you only have to fish out the
crystal once, minimizing handling. I usually do this by preparing an
artificial mother liquor solution that has 125% of the final desired
concent
:51 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] effective iodide conc. for SAD data
Dear All,
I have very thin crystals but diffracting. I was not able to handle them easily
for iodide soak. I always lost the crystals during manipulation and other big
crystals obtained after seeding doesn&#
Hi Rajesh,
it can be a bit all over the place:
For quick soaks, we typically use 500mM-1000mM. A good starting point might be
to simply replace the NaCl concentration in your protein buffer. By some
serendipity we also managed to solve a structure by I/S SAD after a 1mM NaI
soak. One iodide had
Dear All,
I have very thin crystals but diffracting. I was not able to handle them easily
for iodide soak. I always lost the crystals during manipulation and other big
crystals obtained after seeding doesn't even give any diffraction. I tried for
co-crystallizing with NaI. The crystals appear o
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