Did you locate the Iodine(s)? Did you have iodotyrosine, or I^- bound
at anion binding sites? There are two distinct methods based on I2/I-.
Florian Sauer wrote:
Dear Rajesh,
another method to incorporate Iodine into your crystal is by simply
placing a drop of KI/I2 solution next to the crystallization drop.
Have a look here:
Acta Cryst. (2006). D62, 280-289
New methods to prepare iodinated derivatives by vaporizing iodine
labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL)
Worked for me with salt concentration sensitive crystal and a
derivatization time <10 min.
Good luck,
Florian
Am 03.05.12 15:51, schrieb Rajesh Kumar:
Dear All,
I have very thin crystals but diffracting. I was not able to handle
them easily for iodide soak. I always lost the crystals during
manipulation and other big crystals obtained after seeding doesn't
even give any diffraction. I tried for co-crystallizing with NaI. The
crystals appear only up to 20 mM in 1:2 (3ul drop of 1 ul protein and
2ul reservoir).
Is this concentration of iodide is enough for SAD data ( if it had
good incorporation) ?
I appreciate your help.
Thanks
Rajesh
