Yes I did and could solve the structure by SAD. In total 8 I atoms found. 6 bound at anion binding sites. 2 as I2. None covalently bound to tyrosine.
Florian Am 03.05.12 19:42, schrieb Edward A. Berry: > Did you locate the Iodine(s)? Did you have iodotyrosine, or I^- bound > at anion binding sites? There are two distinct methods based on I2/I-. > > Florian Sauer wrote: >> Dear Rajesh, >> >> another method to incorporate Iodine into your crystal is by simply >> placing a drop of KI/I2 solution next to the crystallization drop. >> >> Have a look here: >> >> Acta Cryst. (2006). D62, 280-289 >> New methods to prepare iodinated derivatives by vaporizing iodine >> labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL) >> >> Worked for me with salt concentration sensitive crystal and a >> derivatization time <10 min. >> >> Good luck, >> >> Florian >> >> >> >> >> Am 03.05.12 15:51, schrieb Rajesh Kumar: >>> Dear All, >>> >>> I have very thin crystals but diffracting. I was not able to handle >>> them easily for iodide soak. I always lost the crystals during >>> manipulation and other big crystals obtained after seeding doesn't >>> even give any diffraction. I tried for co-crystallizing with NaI. The >>> crystals appear only up to 20 mM in 1:2 (3ul drop of 1 ul protein and >>> 2ul reservoir). >>> Is this concentration of iodide is enough for SAD data ( if it had >>> good incorporation) ? >>> I appreciate your help. >>> >>> Thanks >>> Rajesh >> >
