Dear All,
I really thank everyone who gave me excellent suggestions.
I will try them to see which one works better for me and my crystals.
Again thanks a lot for your help.
Regards,
Rajesh
Date: Thu, 3 May 2012 20:56:21 +0200
From: florian.sa...@embl-hamburg.de
Subject: Re: [ccp4bb] effective iodide conc. for SAD data
To: CCP4BB@JISCMAIL.AC.UK
Yes I did and could solve the structure by SAD.
In total 8 I atoms found. 6 bound at anion binding sites. 2 as I2.
None covalently bound to tyrosine.
Florian
Am 03.05.12 19:42, schrieb Edward A. Berry:
> Did you locate the Iodine(s)?
Did you have iodotyrosine, or I^- bound
> at anion binding sites? There are two distinct methods based
on I2/I-.
>
> Florian Sauer wrote:
>> Dear Rajesh,
>>
>> another method to incorporate Iodine into your crystal is
by simply
>> placing a drop of KI/I2 solution next to the
crystallization drop.
>>
>> Have a look here:
>>
>> Acta Cryst. (2006). D62, 280-289
>> New methods to prepare iodinated derivatives by
vaporizing iodine
>> labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL)
>>
>> Worked for me with salt concentration sensitive crystal
and a
>> derivatization time <10 min.
>>
>> Good luck,
>>
>> Florian
>>
>>
>>
>>
>> Am 03.05.12 15:51, schrieb Rajesh Kumar:
>>> Dear All,
>>>
>>> I have very thin crystals but diffracting. I was not
able to handle
>>> them easily for iodide soak. I always lost the
crystals during
>>> manipulation and other big crystals obtained after
seeding doesn't
>>> even give any diffraction. I tried for
co-crystallizing with NaI. The
>>> crystals appear only up to 20 mM in 1:2 (3ul drop of
1 ul protein and
>>> 2ul reservoir).
>>> Is this concentration of iodide is enough for SAD
data ( if it had
>>> good incorporation) ?
>>> I appreciate your help.
>>>
>>> Thanks
>>> Rajesh
>>
>
