Re: [ccp4bb] drops swelling

2011-09-10 Thread Ed Pozharski
On Sat, 2011-09-10 at 15:00 +0800, anita p wrote: > Would it be worth while to try and remove glycerol at the last step > which is gel filtration and then set trays in my case? Sure, assuming that your protein does not become unstable without glycerol (protein solubility is likely to decrease too

Re: [ccp4bb] drops swelling

2011-09-10 Thread anita p
Thanks a lot for your suggestions. Would it be worth while to try and remove glycerol at the last step which is gel filtration and then set trays in my case? I have never tried without glycerol. reg. Anita On Fri, Sep 9, 2011 at 7:54 PM, Enrico Stura wrote: > Anita, > > see the message I posted

Re: [ccp4bb] drops swelling

2011-09-09 Thread Enrico Stura
Anita, see the message I posted yesterday on the CCP4bb: http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg22638.html or google "stura glycerol" Re: [ccp4bb] How to help crystal grow bigger In your case the sentence you need is: "You need to have a higher (double) glycerol concentration in t

Re: [ccp4bb] drops swelling

2011-09-09 Thread George T. DeTitta
orge Sent via BlackBerry by AT&T -Original Message- From: anita p Sender: CCP4 bulletin board Date: Fri, 9 Sep 2011 19:22:53 To: Reply-To: anita p Subject: [ccp4bb] drops swelling Dear Crystallographers, I have set hanging drop trays with 2ul of protein and 2 ul of resorvior solutio

Re: [ccp4bb] drops swelling

2011-09-09 Thread David Roberts
The following is simply a quote from a recent post by Enrico Stura. Probably has a lot to do with what you are finding: "Glycerol is also great to reduce nucleation. If you decide to add glycerol to the protein solution (for solubility, but in your case it might be for stability reasons), you

Re: [ccp4bb] drops swelling

2011-09-09 Thread Poul Nissen
Yes - add glycerol to the reservoir - 15% glycerol has a huge influence on the vapour diffusion gradients Poul On 09/09/2011, at 13.22, anita p wrote: > Dear Crystallographers, > I have set hanging drop trays with 2ul of protein and 2 ul of resorvior > solution. I have seen in some cases the

[ccp4bb] drops swelling

2011-09-09 Thread anita p
Dear Crystallographers, I have set hanging drop trays with 2ul of protein and 2 ul of resorvior solution. I have seen in some cases the drops are swelling. My protein buffer has 15% glycerol in it. This is happening mainly when I have peg 400 or peg MME or MPD or Jeffamine in the buffer condition.