Anita,
see the message I posted yesterday on the CCP4bb:
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg22638.html
or google "stura glycerol"
Re: [ccp4bb] How to help crystal grow bigger
In your case the sentence you need is:
"You need to have a higher (double) glycerol concentration in the
reservoir else you will risk finding that your drops will get biggger and
not smaller. This note of caution applies to vapour diffusion set ups as
equilibration can be tricky in such context: Vera,L., Czarny, B.,
Georgiadis, D., Dive, V., Stura, E.A. (2011) Practical Use of Glycerol in
Protein Crystallization. Cryst. Growth & Des. 11 :2755–2762. "
http://pubs.acs.org/doi/abs/10.1021/cg101364m
Enrico.
On Fri, 09 Sep 2011 13:22:53 +0200, anita p <crystals...@gmail.com> wrote:
Dear Crystallographers,
I have set hanging drop trays with 2ul of protein and 2 ul of resorvior
solution. I have seen in some cases the drops are swelling. My protein
buffer has 15% glycerol in it.
This is happening mainly when I have peg 400 or peg MME or MPD or
Jeffamine
in the buffer condition.
Could any one suggest a remedy for this.
with regards
Anita
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
