Yes - add glycerol to the reservoir - 15% glycerol has a huge influence on the vapour diffusion gradients Poul
On 09/09/2011, at 13.22, anita p <crystals...@gmail.com> wrote: > Dear Crystallographers, > I have set hanging drop trays with 2ul of protein and 2 ul of resorvior > solution. I have seen in some cases the drops are swelling. My protein buffer > has 15% glycerol in it. > This is happening mainly when I have peg 400 or peg MME or MPD or Jeffamine > in the buffer condition. > Could any one suggest a remedy for this. > > with regards > Anita
