Hi Yi Zhang
As Sharan suggests microseeding may be the key to solving your problem.
‘Random’ microseeding or MMS, where seed stock is added to random screens
may be helpful. It often gives new hits, crystals that diffract better and
may allow you to control the number of crystals per drop (if you
Dear Jun
Did you tried seeding the old crystals. Just crush them and make different
dilutions with same buffer. Hope, it will work out for you.
Regards
Sharan
Fri, Mar 22, 2019, 6:55 AM 马俊 wrote:
> Dear all,
>A single positive stranded RNA virus RDRP grows crystals, monomers,
> precipita
Dear all,
A single positive stranded RNA virus RDRP grows crystals, monomers,
precipitants (PEG8000)
with low concentration, more nucleuses and poor resolution.Reduce precipitant
concentration can reduce the crystal nucleus, but worse resolution. Precipitant
concentration increased, many cr
