Dear Jun Did you tried seeding the old crystals. Just crush them and make different dilutions with same buffer. Hope, it will work out for you.
Regards Sharan Fri, Mar 22, 2019, 6:55 AM 马俊 <majun...@cau.edu.cn> wrote: > Dear all, > A single positive stranded RNA virus RDRP grows crystals, monomers, > precipitants (PEG8000) > > with low concentration, more nucleuses and poor resolution.Reduce > precipitant concentration can reduce the crystal nucleus, but worse > resolution. Precipitant concentration increased, many crystal nucleus, > crystal is very small. Try to optimize the temperature, pH, different > protein concentration, dehydration, additives, detergent, sit and hanging > drop, but were not improved much. Pease provide some advice methods. > > Thanks > > Jun Ma > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
