Dear all, A single positive stranded RNA virus RDRP grows crystals, monomers, precipitants (PEG8000)
with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution. Precipitant concentration increased, many crystal nucleus, crystal is very small. Try to optimize the temperature, pH, different protein concentration, dehydration, additives, detergent, sit and hanging drop, but were not improved much. Pease provide some advice methods. Thanks Jun Ma ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
