Re: [ccp4bb] Unusual Difference Fourier near Methionines

2007-04-13 Thread Ethayathulla Abdulsamath
l Molecular Biology >University of Leeds, Leeds, LS2 9JT >Telephone: 0113 343 3031 > > -Original Message- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On >Behalf Of Ethayathulla Abdulsamath > Sent: Fri 13 Apr 2007 10:51 > To: [EMA

Re: [ccp4bb] Unusual Difference Fourier near Methionines

2007-04-13 Thread Eleanor Dodson
I think the SD-CE is pointing the wrong way. And they look much too close. I would rotate them and re-refine Eleanor PS - any noise on the 3 fold axis will be multiplied by a factor of 3 Ethayathulla Abdulsamath wrote: Dear all I am doing one structure at 2.6A resolution where I found unu

Re: [ccp4bb] Unusual Difference Fourier near Methionines

2007-04-13 Thread artem
This appears to be a symmetry threefold axis. These areas are notoriously prone to have density artefacts. Also it looks like your terminal carbons are pointing in the opposite way, and the entire end of Met is slightly shifted as a result. You may never be able to fit this area to your satisfactio

Re: [ccp4bb] Unusual Difference Fourier near Methionines

2007-04-13 Thread Thomas Edwards
: [ccp4bb] Unusual Difference Fourier near Methionines Dear all I am doing one structure at 2.6A resolution where I found unusual density near methionines. Actually three methionines come close together nearby and I get difference even at 5sigma

Re: [ccp4bb] Unusual Difference Fourier near Methionines

2007-04-13 Thread David Schwefel
[EMAIL PROTECTED] Betreff: [ccp4bb] Unusual Difference Fourier near Methionines > > Dear all > > I am doing one structure at 2.6A resolution where I found unusual density > near methionines. Actually three methionines come close together nearby and > I get difference even a