Re: [ccp4bb] Strange spots

Dear All, I posted some odd diffraction late last year consisting of Bragg diffraction spots with a diffuse ring or halo. Along with Richard Welberry at ANU we have now published an explanation for this diffuse scattering. For those that are interested the reference is:- Acta Cryst. (2011).

Re: [ccp4bb] Strange spots

ginal Message- > From: Jacob Keller [mailto:j-kell...@fsm.northwestern.edu] > Sent: 04 November 2010 00:57 > To: Nave, Colin (DLSLtd,RAL,DIA); CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Strange spots > > I don't think it looks like satellite spots at all--it looks > more li

Re: [ccp4bb] Strange spots

03, 2010 12:52 PM Subject: Re: [ccp4bb] Strange spots OK! John prompted me to look more carefully at the images and they don't seem to be consistent with any optics or detector effect. Attached a Blowup (almost as strange as Antonioni's 1966 film with this name) of one of the areas. As o

Re: [ccp4bb] Strange spots

elliwell > Sent: 03 November 2010 17:00 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Strange spots > > Dear David, > Many thanks indeed for this movie and the extra info. > It is quite captivating! > > The 'strange spot' features do seem progress

Re: [ccp4bb] Strange spots

ubject: Re: [ccp4bb] Strange spots Dear All, Thank you for the replies sorry about the delay in my reply. Here is some more information, for those of you that are interested, to try fill in some gaps. The data was collected on our home source with osmic vairmax-HF optics and an RAXISIV++ detect

Re: [ccp4bb] Strange spots

Dear David, Many thanks indeed for this movie and the extra info. It is quite captivating! The 'strange spot' features do seem progress to other regions of reciprocal space at approximately constant diffraction resolution in an anti-clockwise manner.but I am still digesting your movie Beh

Re: [ccp4bb] Strange spots

Dear All, Thank you for the replies sorry about the delay in my reply. Here is some more information, for those of you that are interested, to try fill in some gaps. The data was collected on our home source with osmic vairmax-HF optics and an RAXISIV++ detector. We are investigating whether

Re: [ccp4bb] Strange spots

P4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On > Behalf Of John R Helliwell > Sent: 31 October 2010 12:47 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Strange spots > > Dear Jurgen and Petr, > I looked at the Princeton incommensurate link kindly provided by Petr. >

Re: [ccp4bb] Strange spots

Dear Jurgen and Petr, I looked at the Princeton incommensurate link kindly provided by Petr. I see your point ie examples of a grouping of subsidiary spots around a central spot. But not continuous circles. I have now checked the Atlas of Optical Transforms (my office copy en route to Manchester

Re: [ccp4bb] Strange spots

Fig from John (I have a copy of his book in the office and will see it only tomorrow) due to better quality makes it clear (at least for me) that we see the similar effect shown by David Goldstone. Dr Felix Frolow Professor of Structural Biology and Biotechnology Department of Molecular Micro

Re: [ccp4bb] Strange spots - In book of John Helliwell

thing like this before! I am >>> watching this thread with interest to see what others suggest. >>> >>> THanks Also thanks should go specifically to Julian Nomme who took the >>> trouble to send us all the Helliwell book link. >>>

Re: [ccp4bb] Strange spots

ie. From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of James Holton Sent: Friday, October 29, 2010 7:04 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Strange spots The shape reminds me of the focused beam spot profile from slightly misaligned capillary optics. I don&

Re: [ccp4bb] Strange spots

The shape reminds me of the focused beam spot profile from slightly misaligned capillary optics. I don't have too much experience with these, but the incident beam profile does tend to "propagate" through to the spots on the detector. I wonder, could what you are seeing be a "halo" of minor incid

Re: [ccp4bb] Strange spots

to send us all the Helliwell book link. Liz From: CCP4 bulletin board on behalf of Sanishvili, Ruslan Sent: Fri 29/10/2010 21:08 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Strange spots C'mon now! Everybody knows that frogs in real space become hands

Re: [ccp4bb] Strange spots

Are these very strong reflections? Do they appear on more than one image? Are they an artefact of the detector or the image display program?

Re: [ccp4bb] Strange spots

Hi Dave, The circles are quite prominent in the "inner circle (lune)" that you highlight but not in the next one. The full image is too small to see details but I don't see any clear circular halos for any of the other lunes. If you start with the lune going through the origin and number it z

Re: [ccp4bb] Strange spots

le to send us all the Helliwell book link. > > Liz > > > > From: CCP4 bulletin board on behalf of Sanishvili, Ruslan > Sent: Fri 29/10/2010 21:08 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Strange spots > > > >

Re: [ccp4bb] Strange spots

On Fri, Oct 29, 2010 at 4:25 PM, Ethan Merritt wrote: > You may laugh, but the Google finds hits on the topic here: > >   http://atlas-conferences.com/cgi-bin/abstract/cauu-47 anyone who made complete sense of that abstract is invited to go here : http://snarxiv.org/vs-arxiv/ and if you do, ple

Re: [ccp4bb] Strange spots

Autoindexing in the truest sense of the word? ;-) On 10/29/10 12:08 PM, "David Goldstone" wrote: Dear All, Does anyone have any insight into what the circles around the spots might be? cheers Dave -- David Goldstone, PhD National Institute for Medical Research Molecular Structure The Ridgewa

Re: [ccp4bb] Strange spots

On Friday, October 29, 2010 12:59:40 pm Jacob Keller wrote: > > Yes, but the question is what in real space gives rise to reciprocal-space > frog spawn? (Frogs, I guess?) You may laugh, but the Google finds hits on the topic here: http://www.ph.surrey.ac.uk/newsite/ugrad_uploads/Lisowski2004

Re: [ccp4bb] Strange spots

From: CCP4 bulletin board on behalf of Sanishvili, Ruslan Sent: Fri 29/10/2010 21:08 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Strange spots C'mon now! Everybody knows that frogs in real space become handsome princes in the reciprocal one... N. R

Re: [ccp4bb] Strange spots

I think this is a poly-crystalline incommensurately modulated crystal, i.e. incommensurately modulated crystal, which fractured upon freezing, resulting in averaging of satellite spots. Fig. 3b from here: http://www.princeton.edu/~actin/documents/Proteincrystalscanbeincommensurate lymodulated.pdf

Re: [ccp4bb] Strange spots

Only when you do refinement(explains other thread about the unexplained R-gap) JPK - Original Message - From: Sanishvili, Ruslan To: Jacob Keller ; CCP4BB@JISCMAIL.AC.UK Sent: Friday, October 29, 2010 3:08 PM Subject: RE: [ccp4bb] Strange spots C'mon now! Ever

Re: [ccp4bb] Strange spots

l.gov From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jacob Keller Sent: Friday, October 29, 2010 3:00 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Strange spots Yes, but the question is what in real space gives rise to reciprocal-space frog spawn? (Fro

Re: [ccp4bb] Strange spots

Yes, but the question is what in real space gives rise to reciprocal-space frog spawn? (Frogs, I guess?) - Original Message - From: Marcus Winter To: CCP4BB@JISCMAIL.AC.UK Sent: Friday, October 29, 2010 2:56 PM Subject: Re: [ccp4bb] Strange spots Dear

Re: [ccp4bb] Strange spots

Sent: 29 October 2010 17:08 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Strange spots Dear All, Does anyone have any insight into what the circles around the spots might be? cheers Dave -- David Goldstone, PhD National Institute for Medical Research Molecular Structure The Ridgeway

Re: [ccp4bb] Strange spots

spot. Best- Steve -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Julian Nomme Sent: Friday, October 29, 2010 2:10 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Strange spots Google books: http://books.google.com/books?id=RqNb241Q484C&pr

Re: [ccp4bb] Strange spots

Google books: http://books.google.com/books?id=RqNb241Q484C&printsec=frontcover&dq=Macromolecular+Crystallography+with+Synchrotron+Radiation&hl=en&ei=JA3LTMe2NMSnnQfAjN3mDw&sa=X&oi=book_result&ct=result&resnum=1&ved=0CC8Q6AEwAA#v=onepage&q&f=false

Re: [ccp4bb] Strange spots

Dear Gerard I will do a scan of fig 8.1b asap, probably Monday. Greetings, John Sent from my iPad On 29 Oct 2010, at 18:44, Gerard Bricogne wrote: > Dear John, > > Would it be possible to know more about what you are referring to > without having to buy (or steal) your book :-)) ? > >

Re: [ccp4bb] Strange spots

Dear John, Would it be possible to know more about what you are referring to without having to buy (or steal) your book :-)) ? Thank you in advance! With best wishes, Gerard. -- On Fri, Oct 29, 2010 at 06:41:51PM +0100, John R Helliwell wrote: > Dear Da

Re: [ccp4bb] Strange spots

Dear Dave, You have a collector's item there! The closest I have seen is illustrated in my book 'Macromolecular Crystallography with Synchrotron Radiation' page 321, which is a small molecule example. Best wishes, John Prof John R Helliwell DSc On Fri, Oct 29, 2010 at 5:08 PM, David Goldstone wr