Dear All,
Thank you for the replies sorry about the delay in my reply. Here is
some more information, for those of you that are interested, to try fill
in some gaps.
The data was collected on our home source with osmic vairmax-HF optics
and an RAXISIV++ detector. We are investigating whether it is an optics
issue but this is unlikely as other crystals in the screening run didn't
display this phenomenon.
The crystal was grown in the presence of 12% glycerol and transfered to
20% glycerol as a cryo. I haven't tried other cryos as crystals also
grow in 20% glycerol and do not require further cryoprotectant.
I have uploaded a movie showing a wedge of data showing how the circles
around the spots progress.
http://www.4shared.com/video/o8_YmInD/Spot_defect.html (~12mb download)
The crystals index and scale in p6122 (a=b=73, c=110) with a single
monomer in the ASU (by matthews, 45% solvent). We do however see a peak
in the native patterson at (0,0,0.2 ~50% origin height).
Cheers
Dave
On 29/10/2010 17:08, David Goldstone wrote:
Dear All,
Does anyone have any insight into what the circles around the spots
might be?
cheers
Dave
--
David Goldstone, PhD
National Institute for Medical Research
Molecular Structure
The Ridgeway
Mill Hill
London NW7 1AA
