Is it known/can you divulge the nature/structure of the macromolecules in
the crystal? Is it protein, nucleic acid, other? Does the structure have
periodicity to it?
JPK
----- Original Message -----
From: "David Goldstone" <david.goldst...@nimr.mrc.ac.uk>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Wednesday, November 03, 2010 10:53 AM
Subject: Re: [ccp4bb] Strange spots
Dear All,
Thank you for the replies sorry about the delay in my reply. Here is some
more information, for those of you that are interested, to try fill in
some gaps.
The data was collected on our home source with osmic vairmax-HF optics and
an RAXISIV++ detector. We are investigating whether it is an optics issue
but this is unlikely as other crystals in the screening run didn't display
this phenomenon.
The crystal was grown in the presence of 12% glycerol and transfered to
20% glycerol as a cryo. I haven't tried other cryos as crystals also grow
in 20% glycerol and do not require further cryoprotectant.
I have uploaded a movie showing a wedge of data showing how the circles
around the spots progress.
http://www.4shared.com/video/o8_YmInD/Spot_defect.html (~12mb download)
The crystals index and scale in p6122 (a=b=73, c=110) with a single
monomer in the ASU (by matthews, 45% solvent). We do however see a peak in
the native patterson at (0,0,0.2 ~50% origin height).
Cheers
Dave
On 29/10/2010 17:08, David Goldstone wrote:
Dear All,
Does anyone have any insight into what the circles around the spots
might be?
cheers
Dave
--
David Goldstone, PhD
National Institute for Medical Research
Molecular Structure
The Ridgeway
Mill Hill
London NW7 1AA
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
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lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
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