Dear David, Many thanks indeed for this movie and the extra info. It is quite captivating!
The 'strange spot' features do seem progress to other regions of reciprocal space at approximately constant diffraction resolution in an anti-clockwise manner.....but I am still digesting your movie.... Behind the scenes discussion, between Colin Nave, James Holton and myself, has been going on. Since Colin has been the main leader in these I leave it to Colin to take it up from here and I can chip in if I can add anything. Greetings, John On Wed, Nov 3, 2010 at 3:53 PM, David Goldstone <david.goldst...@nimr.mrc.ac.uk> wrote: > Dear All, > > Thank you for the replies sorry about the delay in my reply. Here is some > more information, for those of you that are interested, to try fill in some > gaps. > > The data was collected on our home source with osmic vairmax-HF optics and > an RAXISIV++ detector. We are investigating whether it is an optics issue > but this is unlikely as other crystals in the screening run didn't display > this phenomenon. > > The crystal was grown in the presence of 12% glycerol and transfered to 20% > glycerol as a cryo. I haven't tried other cryos as crystals also grow in 20% > glycerol and do not require further cryoprotectant. > > I have uploaded a movie showing a wedge of data showing how the circles > around the spots progress. > http://www.4shared.com/video/o8_YmInD/Spot_defect.html (~12mb download) > > The crystals index and scale in p6122 (a=b=73, c=110) with a single monomer > in the ASU (by matthews, 45% solvent). We do however see a peak in the > native patterson at (0,0,0.2 ~50% origin height). > > Cheers > > Dave > > > On 29/10/2010 17:08, David Goldstone wrote: >> >> Dear All, >> >> Does anyone have any insight into what the circles around the spots >> might be? >> >> cheers >> >> Dave > > -- > David Goldstone, PhD > National Institute for Medical Research > Molecular Structure > The Ridgeway > Mill Hill > London NW7 1AA > -- Professor John R Helliwell DSc
