Hello
Thanks for your replies and help!
Best regards
Rakesh
On Wed, Jul 28, 2021, 15:58 Julie Tucker wrote:
> Dear Rakesh,
> Congratulations that you have crystals in the first place!
> I am sure you will hear this from others as well, but you did not tell us
> how you tested your crystals for
Dear Rakesh,
Congratulations that you have crystals in the first place!
I am sure you will hear this from others as well, but you did not tell us
how you tested your crystals for diffraction. It is not uncommon that
crystals diffract beautifully in their native environment of the
crystallisation dr
: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Problems with crystals diffracting
Hi everyone
I was working on a protein complex where one protein binds with the other
protein majorly based on its surface charge. The protein complex yields nice
crystals but does not diffract. Additionally the crystals
Hey Rakesh,
Such are the questions that every crystallographer faces in his/her
everyday life. I am not sure if there is any straight forward answer. So
make voyages !
Terese Bergfors mentioned in her famous book, "Protein crystallization" a
case where a Scientist from Umea Uni got the diffraction
Have you tried including a small amount of chymotrypsin? There were quite a few
papers about this technique 10-15 years ago. It worked for one of our proteins,
but I'm not sure about its success with complexes.
Good luck, Jon.C.
Sent from ProtonMail mobile
Original Message
On
Hi everyone
I was working on a protein complex where one protein binds with the other
protein majorly based on its surface charge. The protein complex yields
nice crystals but does not diffract. Additionally the crystals used to
dissolve within the drop when allowed for incubation both at 18 deg
