Have you tried including a small amount of chymotrypsin? There were quite a few papers about this technique 10-15 years ago. It worked for one of our proteins, but I'm not sure about its success with complexes.
Good luck, Jon.C. Sent from ProtonMail mobile -------- Original Message -------- On 27 Jul 2021, 16:54, Rakesh Chatterjee wrote: > Hi everyone > > I was working on a protein complex where one protein binds with the other > protein majorly based on its surface charge. The protein complex yields nice > crystals but does not diffract. Additionally the crystals used to dissolve > within the drop when allowed for incubation both at 18 degrees and 4 degrees. > Repeated rMMS microseeding, construct variations and changes in purification > strategies did not yield any diffractions. Conditions involved for the > protein complexes primarily involved PEG with various strengths and pH > variations. Looking for your suggestions and valuable ideas > > Thanks in advance > > Dr Rakesh Chatterjee > Umea University > Umea Sweden > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
