Hello Thanks for your replies and help!
Best regards Rakesh On Wed, Jul 28, 2021, 15:58 Julie Tucker <julie.tuc...@york.ac.uk> wrote: > Dear Rakesh, > Congratulations that you have crystals in the first place! > I am sure you will hear this from others as well, but you did not tell us > how you tested your crystals for diffraction. It is not uncommon that > crystals diffract beautifully in their native environment of the > crystallisation drop, but then cease to diffract upon transfer into > cryoprotectant and subsequent cryo-cooling. There are many threads on the > bulletin board already dealing with ways to solve this problem, so I will > not go into details here, but will simply refer you to this excellent set > of slides on protein crystal cryocooling from Elspeth Garman: > > https://www.ccp4.ac.uk/schools/DLS-2017/course_material/day1/EGarman_Cryo-cooling.pdf > Good luck! > Julie > > On Tue, 27 Jul 2021 at 16:55, Rakesh Chatterjee <rakesh.2665...@gmail.com> > wrote: > >> Hi everyone >> >> I was working on a protein complex where one protein binds with the other >> protein majorly based on its surface charge. The protein complex yields >> nice crystals but does not diffract. Additionally the crystals used to >> dissolve within the drop when allowed for incubation both at 18 degrees and >> 4 degrees. Repeated rMMS microseeding, construct variations and changes in >> purification strategies did not yield any diffractions. Conditions involved >> for the protein complexes primarily involved PEG with various >> strengths and pH variations. Looking for your suggestions and valuable ideas >> >> Thanks in advance >> Dr Rakesh Chatterjee >> Umea University >> Umea Sweden >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > > > -- > Julie Tucker > York Biomedical Research Institute > Department of Biology and HYMS > University of York > > *Currently working remotely and contactable by e-mail only. Please be > patient if you do not hear from me promptly. I choose to work flexibly; > please only respond to this message when convenient for you to do so.* > > Email disclaimer > <https://www.york.ac.uk/about/legal-statements/email-disclaimer/> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
