Dear Rakesh, Congratulations that you have crystals in the first place! I am sure you will hear this from others as well, but you did not tell us how you tested your crystals for diffraction. It is not uncommon that crystals diffract beautifully in their native environment of the crystallisation drop, but then cease to diffract upon transfer into cryoprotectant and subsequent cryo-cooling. There are many threads on the bulletin board already dealing with ways to solve this problem, so I will not go into details here, but will simply refer you to this excellent set of slides on protein crystal cryocooling from Elspeth Garman: https://www.ccp4.ac.uk/schools/DLS-2017/course_material/day1/EGarman_Cryo-cooling.pdf Good luck! Julie
On Tue, 27 Jul 2021 at 16:55, Rakesh Chatterjee <rakesh.2665...@gmail.com> wrote: > Hi everyone > > I was working on a protein complex where one protein binds with the other > protein majorly based on its surface charge. The protein complex yields > nice crystals but does not diffract. Additionally the crystals used to > dissolve within the drop when allowed for incubation both at 18 degrees and > 4 degrees. Repeated rMMS microseeding, construct variations and changes in > purification strategies did not yield any diffractions. Conditions involved > for the protein complexes primarily involved PEG with various > strengths and pH variations. Looking for your suggestions and valuable ideas > > Thanks in advance > Dr Rakesh Chatterjee > Umea University > Umea Sweden > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > -- Julie Tucker York Biomedical Research Institute Department of Biology and HYMS University of York *Currently working remotely and contactable by e-mail only. Please be patient if you do not hear from me promptly. I choose to work flexibly; please only respond to this message when convenient for you to do so.* Email disclaimer <https://www.york.ac.uk/about/legal-statements/email-disclaimer/> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
