...@jiscmail.ac.uk] On Behalf Of
james09 pruza
Sent: Saturday, October 17, 2009 7:44 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Phase separation to crystals
Dear CCP4bbers,
Surprisingly, only At pH 3.6 the needles are appearing after 3 days but
they are very thin and long rods. I have checked it
What do you define as thin and long rods, for some of us this could be
huge crystals. What's your best guestimate regarding the size ? One
way of approximating this is taking a loop of know size and focus on
it, then focus on the crystal and guess.
Small crystals does not necessarily mean the
ng by robotics, forgot
the name of the company).
Fred.
> Message du 17/10/09 19:44
> De : "james09 pruza"
> A : CCP4BB@JISCMAIL.AC.UK
> Copie à :
> Objet : [ccp4bb] Phase separation to crystals
>
> Dear CCP4bbers,
>
> Surprisingly, only At pH 3.6 the needles
Dear CCP4bbers,
Surprisingly, only At pH 3.6 the needles are appearing after 3 days but
they are very thin and long rods. I have checked it running SDS-PAGE as well
as using IZIT dye . Since the proteins has already 50mM of NaCl so I added
100mM of NaCl in the crystallization mix. The crystal app
Hi James,
I would first ask you what is your crystallization condition?
You mentioned MPD at 70%. Very high concentration (and this is very high) of
organic non volatile alcohols like MPD or polymers like PEGs will promote
phase separation if there is some salt around. The higher the polymer
concen
Dear all,
I need suggestions regarding phase separation. Upon adding NaCl (100mM) in
the crystallization mixture, the full-fledged oiling is observed after 2
days at 4 degree with long needles (pH 3.6). The Protein contains 50mM of
NaCl. What are the general procedures to get the crystals from oil
