Dear CCP4bbers, Surprisingly, only At pH 3.6 the needles are appearing after 3 days but they are very thin and long rods. I have checked it running SDS-PAGE as well as using IZIT dye . Since the proteins has already 50mM of NaCl so I added 100mM of NaCl in the crystallization mix. The crystal appeared at 70% of MPD. Bunch of florets appears without NaCl at the same condition. Will the Additives like Ethanol or 2-propanol help in this condition? Should I not use NaCl in the mix? Changing temperature from 4degree to 25 degree will help? Please advice.
Thanks a lot. James
