Going from 4 to 25 degrees should let you have your crystals faster (if the protein stands that temperature, you should have checked it already). As to improving the crystals, why don't you try the additive kits (several vendors offer these, including some in a form suited for screening by robotics, forgot the name of the company).
Fred. > Message du 17/10/09 19:44 > De : "james09 pruza" > A : CCP4BB@JISCMAIL.AC.UK > Copie à : > Objet : [ccp4bb] Phase separation to crystals > > Dear CCP4bbers, > > Surprisingly, only At pH 3.6 the needles are appearing after 3 days but > they are very thin and long rods. I have checked it running SDS-PAGE as well > as using IZIT dye . Since the proteins has already 50mM of NaCl so I added > 100mM of NaCl in the crystallization mix. The crystal appeared at 70% of > MPD. Bunch of florets appears without NaCl at the same condition. Will the > Additives like Ethanol or 2-propanol help in this condition? Should I not > use NaCl in the mix? Changing temperature from 4degree to 25 degree will > help? > Please advice. > > Thanks a lot. > James >
