Hi
Sodium phosphate monobasic has higher solubility than sodium phosphate
dibasic, while it is other way round for potassium phosphate salts.
Potassium phosphate dibasic is more soluble than potassium phosphate
monobasic. So the best choice is to make solutions of sodium phosphate
monobasic and pot
> Date: Thu, 14 May 2009 09:37:05 -0400
> From: ar...@xtals.org
> Subject: Re: [ccp4bb] Na/K Phosphate
> To: CCP4BB@JISCMAIL.AC.UK
>
> The reason for the odd K/Na combination is solubility - mixed phosphate is
> more soluble than either of the individual ones.
>
Hampton Research has a good chart for preparing Na/K phosphate solutions:
http://hamptonresearch.com/documents/product/23-000180.pdf
Hope this helps!
annie
Annie Hassell
Glaxo Smithkline
5 Moore Drive
RTP, NC 27709
919/483-3228
919/483-0368 (FAX)
annie.m.hass...@gsk.com
> De
The reason for the odd K/Na combination is solubility - mixed phosphate is
more soluble than either of the individual ones.
I try to avoid high phosphate conditions as the Plague. It's great for
molecular biology but horrible for crystallization because phosphate + a
variety of other ions = lovely
Hi,
pure sodium phosphate or pure potassium phosphate are not as soluble as
the mix of both. Especially if you crystallize at temperatures below
20°C you will get a lot of salt crystals.
I used 2.5 M or 2.0 M stocks of Na2HPO4 and KH2PO4 (or the other way
round, i.e.NaH2PO4 and KH22PO4) and ad
Dear all,
Quite a few crystallisation conditions in the screens feature
'sodium/potassium phosphate'. I'm curious to know why such a Na/K mix
is there. As the pH is mostly determined by the (H2PO4)- to (HPO4)2-
ratio, is there such a need to have both cations? If so, is the Na to
K ratio important
