Hampton Research has a good chart for preparing Na/K phosphate solutions: http://hamptonresearch.com/documents/product/0000000023-0000000180.pdf
Hope this helps! annie Annie Hassell Glaxo Smithkline 5 Moore Drive RTP, NC 27709 919/483-3228 919/483-0368 (FAX) annie.m.hass...@gsk.com > Dear all, > > Quite a few crystallisation conditions in the screens feature > 'sodium/potassium phosphate'. I'm curious to know why such a Na/K mix > is there. As the pH is mostly determined by the (H2PO4)- to (HPO4)2- > ratio, is there such a need to have both cations? If so, is the Na to > K ratio important? > > On the more practical side of things, if you want to explore an > initial hit based on sodium/potassium phosphate, how would you go > about it - e.g. what phosphate buffers would you make and at what > ratio would you mix them? > > Thanks in advance, > Geoffrey Kong >
