Dear Mintu,
this seems to be quite a particular case.
Just a side question before we jump on the octet fitting of the 1:3 model.
Do you have any other evidence that your binding partners are binding
in 1:3 stoichiometry? Like a biological mechanism for that or any
other experimental assay like SEC?
Dear Perrakis,
Yes, with a 1:3 binding model, I mean stoichiometry with a sequential
binding model. The sensogram profile that I am getting clearly suggests
that neither it is a 1:1 nor 1:2 stoichiometric binding (at least R-square
and RSS values suggest that it might be a 1:3 stoichiometric bindin
Dear Chandra,
What exactly do you mean by 1:3 or 1:2 binding mode? Is that stoichiometry? Do
you need/want to fit a simple binding model with that stoichiometry, a
sequential model, or a co-operative model?
Graphpad can get you a long way with existing equations when you define the
exact probl
Hello everyone,
I am trying to fit a BLI sensogram curve into a 1:3 binding model but there
is limited scope in the software package (limited to 1:2 binding model) and
therefore not able to fit the curve to get the accurate KD value. I am
trying graphpad prism but not getting enough information on
