Dear Perrakis, Yes, with a 1:3 binding model, I mean stoichiometry with a sequential binding model. The sensogram profile that I am getting clearly suggests that neither it is a 1:1 nor 1:2 stoichiometric binding (at least R-square and RSS values suggest that it might be a 1:3 stoichiometric binding). I can certainly try KINTEK but am currently not familiar with that software.
Thanks. M Chandra On Wed, Aug 28, 2024 at 10:23 AM <a.perra...@nki.nl> wrote: > Dear Chandra, > > What exactly do you mean by 1:3 or 1:2 binding mode? Is that > stoichiometry? Do you need/want to fit a simple binding model with that > stoichiometry, a sequential model, or a co-operative model? > > Graphpad can get you a long way with existing equations when you define > the exact problem, but I am personally very find of the KINTEK software > (you can get a student and trial license for it) which can do anything you > ever want to do with transit and equilibrium kinetics analysis. > > A. > > > > > On 28 Aug 2024, at 17:16, Mintu Chandra <mint...@gmail.com> wrote: > > *LET OP:* Deze e-mail is afkomstig van buiten de organisatie. Open alleen > links of bijlagen als je de afzender kent en weet dat de inhoud veilig is. > > *CAUTION:* This email originated from outside of the organization. Do not > click links or open attachments unless you recognize the sender and know > the content is safe. > Hello everyone, > I am trying to fit a BLI sensogram curve into a 1:3 binding model but > there is limited scope in the software package (limited to 1:2 binding > model) and therefore not able to fit the curve to get the accurate KD > value. I am trying graphpad prism but not getting enough information on > which particular equation I need to use to fit the BLI curve into a 1:3 > binding model. > > Any help or suggestion from the community in this matter would be highly > appreciated. > > Thank you, > M Chandra > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > -- *Mintu Chandra*, PhD Research Assistant Professor Department of Biological Sciences & Biochemistry Vanderbilt University Nashville, TN, USA - 37235 Mobile : 615-947-9274 *Email* : mintu.chan...@vanderbilt.edu *ORCID* : https://orcid.org/0000-0003-2382-5024 *Google Scholar* : https://scholar.google.com.au/citations?user=xYytyGAAAAAJ&hl=en Linkedin : https://www.linkedin.com/in/dr-mintu-chandra-584a8b2b/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
