Dear Chandra, What exactly do you mean by 1:3 or 1:2 binding mode? Is that stoichiometry? Do you need/want to fit a simple binding model with that stoichiometry, a sequential model, or a co-operative model?
Graphpad can get you a long way with existing equations when you define the exact problem, but I am personally very find of the KINTEK software (you can get a student and trial license for it) which can do anything you ever want to do with transit and equilibrium kinetics analysis. A. On 28 Aug 2024, at 17:16, Mintu Chandra <mint...@gmail.com> wrote: LET OP: Deze e-mail is afkomstig van buiten de organisatie. Open alleen links of bijlagen als je de afzender kent en weet dat de inhoud veilig is. CAUTION: This email originated from outside of the organization. Do not click links or open attachments unless you recognize the sender and know the content is safe. Hello everyone, I am trying to fit a BLI sensogram curve into a 1:3 binding model but there is limited scope in the software package (limited to 1:2 binding model) and therefore not able to fit the curve to get the accurate KD value. I am trying graphpad prism but not getting enough information on which particular equation I need to use to fit the BLI curve into a 1:3 binding model. Any help or suggestion from the community in this matter would be highly appreciated. Thank you, M Chandra ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
