Hello everyone,
I am trying to fit a BLI sensogram curve into a 1:3 binding model but there
is limited scope in the software package (limited to 1:2 binding model) and
therefore not able to fit the curve to get the accurate KD value. I am
trying graphpad prism but not getting enough information on which
particular equation I need to use to fit the BLI curve into a 1:3 binding
model.

Any help or suggestion from the community in this matter would be highly
appreciated.

Thank you,
M Chandra

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