As a follow-up, we have a postdoc position open to further develop metal
identification methods for crystals and in solution!
The project: structural study of biological systems that are impacted by free
radical activity and metalloproteins, where the modeled metal and/or
coordination state are
From: CCP4 bulletin board on behalf of Andrew Lovering
Sent: 07 January 2021 14:13
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Elemental Analysis
Dear CCP4ers
We have a protein with moderate resolution (mid 2s) with obvious metal ion
density holding the oligomer together. We
Hi Andy,
We were able to use EDX fluorescence scans at a beam line to detect calcium in
crystals.
https://pubmed.ncbi.nlm.nih.gov/29890074/
It isn't quantitative like micro-pixe but you can see it...
Eddie Snell and Elspeth Garman and team have done some further work on
micro-pixe, as well –
ect: [ccp4bb] Elemental Analysis
Dear CCP4ers
We have a protein with moderate resolution (mid 2s) with obvious metal ion
density holding the oligomer together. We can make an educated guess that the
ions are likely to be Mg/Ca and can look at co-ordination and bond
lengths.but I have a m
s://hwi.buffalo.edu/>
[hwi-logo-primary-horizontal]
From: CCP4 bulletin board On Behalf Of Andrew Lovering
Sent: Thursday, January 7, 2021 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Elemental Analysis
Dear CCP4ers We have a protein with moderate resolution (mid 2s) with obvi
Hello again, re: amount, yes, from memory, it was a few milligrams. We also
used EDAX on dried samples IIRC. Cheers, Jon.C.
Sent from ProtonMail mobile
Original Message
On 7 Jan 2021, 14:13, Andrew Lovering wrote:
> Dear CCP4ers
>
> We have a protein with moderate resolution (
Hello, there is atomic-absorption. Breaks your heart to see the protein going
into the flame, but we got good results for Se and transition metals with a
superoxide dismutase. I doubt if there'll ever be one of those on a beam line
;-0 Cheers, Jon Cooper.
Sent from ProtonMail mobile
O
Dear CCP4ers
We have a protein with moderate resolution (mid 2s) with obvious metal ion
density holding the oligomer together. We can make an educated guess that the
ions are likely to be Mg/Ca and can look at co-ordination and bond
lengths.but I have a more specific question - would it be
