inada"
De: CCP4 bulletin board em nome de 白雪慧
Enviado: segunda-feira, 16 de dezembro de 2024 08:59
Para: CCP4BB@JISCMAIL.AC.UK
Assunto: [ccp4bb] Crystal optimization
Hello, everyone. Thank you for your help earlier.I also found that there is a
strange
Jose
From: CCP4 bulletin board on behalf of 白雪慧
Date: Friday, 31 May 2024 at 03:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal optimization
Some people who received this message don't often get email from
zb20193020...@cau.edu.cn. Learn why this is
important<
do: Friday, May 31, 2024 3:32:52 AM
Para: CCP4BB@JISCMAIL.AC.UK
Assunto: [ccp4bb] Crystal optimization
Thank you very much for your suggestions. I have a question. My crystal grows
microcrystals under multiple conditions, as shown in the figure. After
orthogonal optimization of the precipitant a
Apologies, my response was directly to the OP and not to the BB. I have
forwarded the response to maintain the chain.
-- Forwarded message -
From: Nicholas Clark
Date: Fri, May 31, 2024 at 7:23 AM
Subject: Re: [ccp4bb] Crystal optimization
To: 白雪慧
I had a protein that no
Hi,
In addition, you might want to test additives. I would suggest the additive
screen from Hampton Research. We made best experiences with neutral solvents
like: ethylacetate (doi.org/10.1021/ic010790t and
doi.org/10.1021/acs.inorgchem.6b00672), dioxane
(doi.org/10.1021/acs.inorgchem.6b00672)
and DSF is one way to test. But might need a new construct or
surface mutation or other protein engineering.
Good luck
Frank
Sent from tiny silly touch screen
From: 白雪慧
Sent: Friday, 31 May 2024 03:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal optimizat
seeds to nucleate from.
Best of luck, tom
From: CCP4 bulletin board on behalf of 白雪慧
Sent: Friday, May 31, 2024 12:32 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal optimization
You don't often get email from zb20193020...@cau.edu.cn. Learn why th
ir.com
>>
>> b...@ruppweb.org
>>
>> http://www.vienna-air.com/
>>
>> -
>>
>> It is not your aptitude but your attitude
>>
>> that determines your altitude. (or your crystals)
>>
>> -
*
>
> It is not your aptitude but your attitude
>
> that determines your altitude. (or your crystals)
>
> -
>
> ** **
>
> ** **
>
> ** **
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> yybbll
>
> Sent: Tuesday, July 10, 2012 11:58 AM
>
als)
-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of yybbll
Sent: Tuesday, July 10, 2012 11:58 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal Optimization
Hi,
In my experience, it is very very very difficult to optim
Hi,
In my experience, it is very very very difficult to optimize this needle like
crystal. Always give up.
Good luck!
Dear All;
Could somebody give a nice suggestion how the following type crystal could
be optimized, I almost tried everything.
Crystal Image is attached
Crystal condition: 20%
bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Greg
Costakes
Sent: Tuesday, July 10, 2012 2:18 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal Optimization
Have you tried multiple rounds of micro-seeding
M
Subject: [ccp4bb] Crystal Optimization
Dear All;
Could somebody give a nice suggestion how the following type crystal could
be optimized, I almost tried everything.
Crystal Image is attached
Crystal condition: 20% w/v PEG3350 and 200mM NaCl.
Thanks in advance
Bashir
--
Muhammad B
Give a list of all you have tried?
JPK
On Tue, Jul 10, 2012 at 12:22 PM, Muhammed bashir Khan <
muhammad.bashir.k...@univie.ac.at> wrote:
> Dear All;
>
> Could somebody give a nice suggestion how the following type crystal could
> be optimized, I almost tried everything.
>
> Crystal Image is att
Dear All,
Thank you for the replies to my post. I received more than 14 response. The
summary is given below.
The suggestions were
- add another purification step.
* Ni-column
* remove His-tag
* Ni-column
* gel filtration
if you have done that: add another step to test for better crystals
:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal Optimization
Dear All,
We got crystals for a 35 KDa protein with 323aa including His tag and linker.
It was originally crystallized in 0.1M Bis Tris Propane pH:6.5, 0.2M Potassium
thiocyanate and 20% PEG 3350. Later we managed to obtain
where the traditional 96 reagent
additive screens and the detergent screens do not.
Good Luck!
Annie
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jobichen
Chacko
Sent: Wednesday, April 13, 2011 5:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal Optimization
De
sed by the freezing.
Zhijie
From: Jobichen Chacko
Sent: Wednesday, April 13, 2011 5:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal Optimization
Dear All,
We got crystals for a 35 KDa protein with 323aa including His tag and linker.
It was originally crystallized in 0.1M BisTris
se regular optimization approaches: Additive screen etc. Sometimes
> cleave the tag or change it to another end will work.
>
> Cheers,
>
> Bingfa
>
>
>
> 发件人: owner-ccp...@jiscmail.ac.uk [mailto:owner-ccp...@jiscmail.ac.uk] 代表
> Jobichen Chacko
> 发送时间: 2011年4月13日 17:
: Additive screen etc. Sometimes
cleave the tag or change it to another end will work.
Cheers,
Bingfa
发件人: owner-ccp...@jiscmail.ac.uk [mailto:owner-ccp...@jiscmail.ac.uk] 代表
Jobichen Chacko
发送时间: 2011年4月13日 17:44
收件人: CCP4BB@JISCMAIL.AC.UK
主题: [ccp4bb] Crystal Optimization
Dear All,
We got
Dear Jobi,
The usual suspects are:
- add another purification step. In fact I'd do that first:
* Ni-column
* remove His-tag
* Ni-column
* gel filtration
if you have done that: add another step to test for better crystals
- additive screens
- room temperature data set to check if freezin
Dear All,
We got crystals for a 35 KDa protein with 323aa including His tag and
linker. It was originally crystallized in 0.1M BisTris Propane pH:6.5, 0.2M
Potassium thiocyanate and 20% PEG 3350. Later we managed to obtain crystals
with 0.1M BisTris pH:6.5, 0.2M Potassium thiocyanate and 20% PEG
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