Hi 白雪慧

Initially I would try to diffract these crystals befofe any optimisation. A 
microfocus beam should give you some clues. You might find out that you have a 
super ordered crystal at 1.2 A resolution already, or you crystallized salt or 
a contaminant.

Further, have you tried seeding? These crystals look ideal for it. I would also 
reccommend leaving your plates at cold temperature (10 or 4ºC). This will slow 
down your nucleation events increasing the likelihood of bigger crystals.

Best wishes
____________________________________________________

Rafael Marques da Silva

PhD Student – Structural Biology

University of Leicester

Mestre em Física Biomolecular
Universidade de São Paulo

Bacharel em Ciências Biológicas
Universidade Federal de São Carlos

phone: +44 07861 273773

           "A sorte acompanha uma mente bem treinada"
________________________________________________

________________________________
De: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> em nome de 白雪慧 
<zb20193020...@cau.edu.cn>
Enviado: segunda-feira, 16 de dezembro de 2024 08:59
Para: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Assunto: [ccp4bb] Crystal optimization

Hello, everyone. Thank you for your help earlier.I also found that there is a 
strange phenomenon, want to see how you optimize.The crystal is a cluster with 
many nuclei, but regular crystals, and occasionally single crystals, grow out 
of the holes around the circle.It's in the picture. Have you ever had that 
happen to you?How to optimize?

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