Hi 白雪慧
Initially I would try to diffract these crystals befofe any optimisation. A
microfocus beam should give you some clues. You might find out that you have a
super ordered crystal at 1.2 A resolution already, or you crystallized salt or
a contaminant.
Further, have you tried seeding? These crystals look ideal for it. I would also
reccommend leaving your plates at cold temperature (10 or 4ºC). This will slow
down your nucleation events increasing the likelihood of bigger crystals.
Best wishes
____________________________________________________
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +44 07861 273773
"A sorte acompanha uma mente bem treinada"
________________________________________________
________________________________
De: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> em nome de 白雪慧
<zb20193020...@cau.edu.cn>
Enviado: segunda-feira, 16 de dezembro de 2024 08:59
Para: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Assunto: [ccp4bb] Crystal optimization
Hello, everyone. Thank you for your help earlier.I also found that there is a
strange phenomenon, want to see how you optimize.The crystal is a cluster with
many nuclei, but regular crystals, and occasionally single crystals, grow out
of the holes around the circle.It's in the picture. Have you ever had that
happen to you?How to optimize?
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