Usually, you should try to push up the protein concentration, often quite a lot (30, 50, even 100 mg/ml), and decrease precipitant (might have to be really low, eg <3% PEG is not unthinkable).
To get the protein up, you may need to find a new buffer solution - there are screens for this, and DSF is one way to test. But might need a new construct or surface mutation or other protein engineering. Good luck Frank Sent from tiny silly touch screen ________________________________ From: 白雪慧 <zb20193020...@cau.edu.cn> Sent: Friday, 31 May 2024 03:33 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Crystal optimization Thank you very much for your suggestions. I have a question. My crystal grows microcrystals under multiple conditions, as shown in the figure. After orthogonal optimization of the precipitant and pH, the crystal growth is still very small and difficult to obtain diffraction. What is the method to optimize and increase the crystal size in this situation? ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
