Dear Mike,
At Evotec (a UK site), we gave up using GE to service
our GE instruments (!) due to problems with their bureaucracy. Agilent
offer service contracts for Aktas that are competitively priced and
are a work-alike in our experience.
For call-outs, they sub-contract the usual
@JISCMAIL.AC.UK
Subject: [ccp4bb] Akta Prime
Dear all,
We have an AktaPrime and GE Lifesciences stop servicing these instruments
because they are getting old. Does anyone know of a third party company that
gives contracts to maintain these instruments? Thank you.
Mike Colaneri
I have used and worked on both the Bio-Rad and Akta systems. It is
really a matter of opinion, but they both get the job done.
Exterior:
The Akta systems look nice with metal exteriors while the Bio-Rads look
a little cheap being plastic. Both are really heavy, though.
Interior:
Once you take
As a personal touch, I also find Biorad software much more intuitive.
This discussion, of course, is similar to comparing car brands -
experiences differ.
What mystifies me is a need for a service maintenance contract at all. Has
hardware become so much less reliable than in the past?
Grante
On Wed, 2011-10-12 at 18:18 -0700, Paul Smith wrote:
> and have heard some good things about Bio-rad
Personally, my experience with Biorad was very positive. For instance,
we had the switch valve motor burn out and they sent us the motor for
$150 or so while they could have charged us full price
I know all these because I’ve got a letter from GE regarding my
> AktaPrime.
> >
> > HTH,
> >
> > Vaheh
> >
> >
> >
> > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Paul Smith
> > Sent: Wednesday, October 12, 2011 9:19 PM
&
es anyone have some spare millions and manufacturing connections
> in India/China to consider starting a competing company?
>
> Sorry to hijack your thread Michael. Let me know what you find out. The
> less money I send to GE the better.
>
>
> --Paul
>
>
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Paul Smith
Sent: Wednesday, October 12, 2011 9:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Akta Prime / FPLC Options / Off Topic
Michael,
Unfortunately, I actually don't know who
AC.UK] On Behalf Of Paul Smith
Sent: 13 October 2011 02:19
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Akta Prime / FPLC Options / Off Topic
Michael,
Unfortunately, I actually don't know who serves these machines apart from GE.
Because you brought up the subject of GE equipment and servic
;
> --Paul
>
>
> From: Michael Colaneri
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: Wednesday, October 12, 2011 2:28 PM
> Subject: [ccp4bb] Akta Prime
>
> Dear all,
>
> We have an AktaPrime and GE Lifesciences stop servicing these instrume
Dear Mike,
strange - I just filed a repair job with labcrew for our old ÄKTA Prime. A
technician was in house (for other reasons) when it stopped working and he
was confident that he can replace our pump (rotary unit, as he called
it)... Well let's hope he really does...
On the other hand,
out a service
call. GE probably will support AKTAprime plus for a few more years. --Chun
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Michael Colaneri
Sent: Wednesday, October 12, 2011 11:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Akta Prime
Dear all,
We ha
end to GE the better.
--Paul
From: Michael Colaneri
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, October 12, 2011 2:28 PM
Subject: [ccp4bb] Akta Prime
Dear all,
We have an AktaPrime and GE Lifesciences stop servicing these instruments
because they are getting ol
Dear all,
We have an AktaPrime and GE Lifesciences stop servicing these instruments
because they are getting old. Does anyone know of a third party company
that gives contracts to maintain these instruments? Thank you.
Mike Colaneri
It depends on how your system is configured - primary considerations being
tubing length and tubing inner diameter (AKTA can come with at least three
different PEEK tube diameters, depending on what you purchased it for). New
systems often arrive with at least one tube-change kit (i.e. pre-cut tube
Hi Jim,
If I remember correctly the delay volume from the pumps to the top of the
column is roughly 8 ml for Äkta Purifier, Explorer and also Prime. The actual
volume from the bottom of the column to the fractionator, or more important
from the UV cell to the fractionator depends on the tubing
Hello all,
Does anyone have a rough estimate (or has anyone actually determined) an
average dead/delay volume between buffers run on an AKTA Prime FPLC? We are
attempting to overexpress/isolate a smaller His-tagged transmembrane protein,
and require running several detergent buffers in success
: Thursday, February 15, 2007 12:37 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AKTA prime
Pedant's corner:
The systems are actually called ÄKTA (loosely "the real thing" in Swedish")
and not AKTA ("watch out!", "beware!"), although given the
Pedant's corner:
The systems are actually called ÄKTA (loosely "the real thing" in
Swedish") and not AKTA ("watch out!", "beware!"), although given the
feelings expressed about the price, the latter may not be such a
misnomer ;-)
Phew, I got there before Gerard!
Derek
--
Derek Logan
"David R. Cooper" <[EMAIL PROTECTED]> wrote: From: "David R. Cooper" <[EMAIL
PROTECTED]>
Subject: Re: [ccp4bb] AKTA prime
To: Frank Lee <[EMAIL PROTECTED]>
Date: Wed, 14 Feb 2007 15:13:18 -0500
Hello, Frank.
I seem to be having trouble posting directl
: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AKTA prime
Talking about akta's and beyond... Im offered a 2nd hand Pharmacia FPLC,
but in order to run it with a PC I'd need a copy of FPLCManager. The
software is not sold anymore by Pharmacia --> Amersham --> GE. Would anybody
have a c
Talking about akta's and beyond... Im offered a 2nd hand Pharmacia FPLC,
but in order to run it with a PC I'd need a copy of FPLCManager. The
software is not sold anymore by Pharmacia --> Amersham --> GE. Would anybody
have a copy lying around by any chance?
Thx,
Flip
Hi,
Don't forget system losses (friction in the tubing). An empty AKTA prime
typically eats up about 0.25 MPa so your maximum is closer to 0.7MPa loss
on column.
Artem
>
>
> Dear Frank,
> AKTA prime delivers a max. pressure of 1 MPa. The 24 ml SEC columns can
> be run at pressures
> up to 1.5
Dear Frank,
AKTA prime delivers a max. pressure of 1 MPa. The 24 ml SEC columns can
be run at pressures
up to 1.5 (S200) or even 3 MPa (superose 12). If your column is in pretty
good shape, expect to
reach 0.9 MPa at 0.4 ml/min for S200. So you can use an AKTA prime, but
...
Best
Clemens
At 11:
Dear Frank
AKTAs are excellent chromatographic systems, but you must know that AKTA
prime is not the best choice if you want to do "serious" chromatography.
AKTA prime has only one pump and a valve that alternatively extract
solvent from the two different reservoirs. If you want to do a gradient,
Dear Frank
AKTAs are excellent chromatographic systems, but you must know that AKTA
prime is not the best choice if you want to do "serious" chromatography.
AKTA prime has only one pump and a valve that alternatively extract
solvent from the two different reservoirs. If you want to do a gradient,
Are you sure that you want AKTA anything? They are so expensive.
We have had good service from the old style ("HR") Bio-Rad Bio-Logic
chromatography systems. The new ones are called "DuoFlow".
-Dan
On Tue, 13 Feb 2007, Frank Lee wrote:
>
> Dear all,
>
> I need to decide between buying an AKTA p
Dear all,
I need to decide between buying an AKTA prime and an AKTA FPLC from GE health
care. I understand AKTA prime is a low-pressure system, but because it is too
much cheaper than AKTA FPLC, it is still very attractive to me.
I will mainly use it for Nickel columns and gel filtration colum
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