Hi, Don't forget system losses (friction in the tubing). An empty AKTA prime typically eats up about 0.25 MPa so your maximum is closer to 0.7MPa loss on column.
Artem > > > Dear Frank, > AKTA prime delivers a max. pressure of 1 MPa. The 24 ml SEC columns can > be run at pressures > up to 1.5 (S200) or even 3 MPa (superose 12). If your column is in pretty > good shape, expect to > reach 0.9 MPa at 0.4 ml/min for S200. So you can use an AKTA prime, but > ... > Best > Clemens > > > At 11:16 PM 2/13/2007, you wrote: > > Dear all, > > I need to decide between buying an AKTA prime and an AKTA FPLC from GE > health care. I understand AKTA prime is a low-pressure system, but > because it is too much cheaper than AKTA FPLC, it is still very > attractive to me. > > I will mainly use it for Nickel columns and gel filtration columns, and I > am worried about the latter. Is it true that using AKTA prime, you can > only run the 24 ml Superdex 200 column at 0.1 or 0.2 ml/min? > > Could anyone who has used AKTA prime give me some feedbacks? I would > appreciate it. > > Best, > Frank > > > Need Mail bonding? > Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. > Jun.-Prof. Dr. Clemens Steegborn > Ruhr-University Bochum > Dept. Physiol. Chemistry, MA 2/141 > Universitaetsstr. 150 > 44801 Bochum, Germany > > phone: 0049 234 32 27041 > fax: 0049 234 32 14193 > email: [EMAIL PROTECTED]
