Re: [ccp4bb] Superimposition save as PDB for ccp4 refmac question

Hi Marco, You could use the 'combine' command. You could select two models in chimeraX and which will write a single pdb file. Look at the below link. https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/combine.html Best, Rajiv On Tue, 27 Feb, 2024, 7:54 pm Marco Bravo, wrote: > Hello all

[ccp4bb] Two open postdoctoral positions in Bhubaneswar, India

andhi Sundaram, PhD DBT - Institute of Life Sciences, (an autonomous Institute of the Department of Biotechnology, Ministry of Science & Technology, Govt of India) Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha, PIN: 7510

[ccp4bb] Two postdoctoral positions available

t rsr...@niser.ac.in with your CV. Please help to pass the information to your colleagues. Thank you Best regards, ** *Rajivgandhi Sundaram, PhD,* Dr. Dileep Vasudevan's group DBT - Institute of Life Sciences, (an autonomous Institute of the Dep

Re: [ccp4bb] Manual His-Tag Purification

Thanks everyone! Thanks Prof. David Blum. I will do batch load and check. Yours Sincerely, Sundaram.S On Wed, Feb 1, 2017 at 9:06 PM, David Blum wrote: > Hi Sundaram, > > The binding capacity of this column is 40 mg/mL of resin so a 5mL column > will hold a maximum of 200 mg of

[ccp4bb] Manual His-Tag Purification

Hello , It's an off topic question. I'm planning to do manual purification a 6 his tagged protein of size around 20kDa from 1L E.coli culture using COHISC-RO Roche cOmplete™ His-Tag Purification Column. Can I get some advice regarding the lysate loading volume and retention time. This is the fi

[ccp4bb] Problems with PDB validation

Hi all, I am currently validating my protein and experiencing a few issues. I am unable to fix the torsion angles for eight residues no matter what I try. There are 3 models deposited on the PDB website belonging to the same protein. Five of the residues’ torsion angles (of the eight) are cons

[ccp4bb] Generating low resolution density maps from atomic models

Hi all, Just wanted to ask if anyone has generated low resoultion density maps before? If yes, can anyone help me with this or point me in the right direction? Thanks in advance for your help!

[ccp4bb] Molecular replacement

Hi all, I am working on an oxidoreductase and having some trouble during molecular replacement. The resolution of the crystal is 1.7 A and the space group is I4122 (a = b = 121.086, c =156.93 and alpha = beta = gamma = 90). The cell content analysis results predicted two molecules in the