Hi all,
 
I am working on an oxidoreductase and having some trouble during molecular 
replacement.
 
The resolution of the crystal is 1.7 A and the space group is I4122 (a = b = 
121.086, c =156.93 and alpha = beta = gamma = 90). 
 
The cell content analysis results  predicted two molecules in the asymmetric 
unit and the solvent content as 36 %. The Matthew's coefficient is 1.94. The 
Wilson plot also looks fine.
 
However, after molecular replacement (using Phaser) the result just gives me 
just a single molecule. ( RFZ = 8.5 TFZ =13.9 PAK = 0 LLG =189 TFZ=18.0 LLG = 
642)
 
Any suggestions how to solve this problem?
 
Thanks in advance.
 

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