Hello , It's an off topic question.
I'm planning to do manual purification a 6 his tagged protein of size around 20kDa from 1L E.coli culture using COHISC-RO Roche cOmplete™ His-Tag Purification Column. Can I get some advice regarding the lysate loading volume and retention time. This is the first time I going to use this column and I have no idea about my protein yield from 1L culture. Sorry if I spammed your inbox. Thanks! Yours Sincerely, Sundaram.S
