B-Factors should do the same
thing.
With a large ligand, I suspect that some of the waters will also have
alternate orientations. You also might look at the amino acids that
bind to the ligand to see if there is any alternate conformations.
Best
Steven Herron
On 3/3/2022 8:51 AM, Akanksha T
Fatty acids are very soluble in glycerol or the PEGs.
Most proteins tolerate high concentrations (30-50%) glycerol and PEGs.�
So, it is quite possible to make a solution that will allow the protein
crsytal and the ligand to both be in the same solution.
One of my former colleagues was in ch
hour period of time. If I went faster, the sulfate would remain in
the crystal channels and would precipitate when exposed to calcium. It
was tedious working this out, but it was essential for everything else
that came later.
Steven Herron [sherron_...@yahoo.com]
On 4/23/2021 11:40 PM
/pubmed/7893690
http://pubs.acs.org/doi/pdf/10.1021/bi9a007
When lysine-166 is changed to arginine, one side of the reaction pH
profile shifts to the basic. If I remember correctly, the mutation
causes a 2 pH unit shift of the basic side of the pH reaction profile.
Steven Herron
sherron_
Does the Hauptman-Woodward Medical Research Institute have any sample
data-sets, so we can see what the results look like?
On 4/14/2017 8:58 AM, Edward Snell wrote:
Dear Elizabeth,
The High-Throughput Crystallization Screening Center and the
Hauptman-Woodward Medical Research Institute
sidue
orientation and the Ca2+ ion occupancy.
Steven Herron
sherron_...@yahoo.com
On 5/6/2014 11:02 AM, Chris Fage wrote:
Hi Everyone,
In my 2.5-angstrom structure, there is negative Fo-Fc density
surrounding a metal ion after refining in Phenix. From anomalous
diffraction I am certain of the me
Can you scan the page and submit the image to the bb?
Besides increasing the chance of identifying the source, I am sure I am
not the only one who is curious to see the mystery page.
Steven Herron
On 7/18/2013 9:29 PM, Edward Snell wrote:
Dear All,
On a beamline visit I came across a
David,
Do you know of a program to make accurate braille representations of an
electron density map :)
That would be cool.
Maybe in the future [See:
http://www.wired.com/wiredscience/2012/08/smart-fingertips-virtual-senses/
http://www.ethlife.ethz.ch/archive_articles/100816_virtuelle_realita
Jürgen Quote: "Propane for whatever reason has gone extinct in certain
areas of the world :-) ."
I went to SSRL (Stanford) with a colleague who wanted to use liquid
propane. We had to go through a mound of paper work to get permission
bring propane on site and set up the experiments. I do
The "Race for the Double Helix" (2004)
http://www.amazon.com/Race-Double-Helix-VHS-Pigott-Smith/dp/6303247911/ref=sr_1_1?ie=UTF8&s=dvd&qid=1271365899&sr=1-1
When crystallography was on "FILMS" not CCD's:)
Eva Kirchner wrote:
"favourite movies involving real science"
The Dark Crystal (
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