Dear All,
Thanks for the suggestions.
Jobi
On Tue, 15 Dec 2020 at 21:58, Jobichen Chacko wrote:
> Dear All,
> I accidentally deleted the old version of CCP4I, while installing the
> latest version. I copied the whole directory from another MAC, but unable
> to start the old GUI
Dear All,
I accidentally deleted the old version of CCP4I, while installing the
latest version. I copied the whole directory from another MAC, but unable
to start the old GUI.
Is there anyway to get back the old version?
I find the old GUI handy for running lot of small programs.
Thank you.
Jobi
#
solution, refinement and validation of
macromolecular crystallography data using various software in the CCP4
program suite.
For details and registration please see the below link.
https://asca2019.org/programme-at-a-glance/
Contact Person: Dr Jobichen Chacko
Email: jobich
Dear Benjamin,
Have you tried the PDBsum website?
http://www.ebi.ac.uk/thornton-srv/databases/pdbsum/Generate.html
It can generate the topology diagram.
Thank you.
Jobi
Sent from my iPhone
> On 21 Jan 2019, at 6:08 PM, Benjamin Butt wrote:
>
> Hi all,
>
> I am trying to generate a topology di
Dear All,
I am trying to connect to these two servers, but it seems like not working.
http://tanna.bch.ed.ac.uk/index.html
http://eduliss.bch.ed.ac.uk/MESPEUS/
Any alternate server that provides the same information?
Thank you.
Jobi
##
Dear All,
We are trying to identify/sequence a DNA/RNA fragment (around 100bp) which
was co-purified along with our protein.
The expression was done in E.coli.
Any suggestions on how to do this.
Thank you.
Jobi
Dear All,
Any solution to this problem.
I am also encountering this issue with MacOS, managed to revert back
from the latest CCP4 update and it is working fine.
Thanks.
Jobi
On Sat, Dec 10, 2016 at 2:42 AM, David Waterman wrote:
> Dear Oliver,
>
> Thanks for digging deeper. I don't know why this
Dear All,
I am running a molecular replacement using MRage program for almost 1 week
with 4 threads, but yet to finish the run. I am using Phenix Version
dev-1549. I am having a pretty big protein (around 100 KDa).
I gave data file, sequence file, one model pdb and output from HHpred as
input.
Is
Dear All,
I am looking for buying some metal clusters like TAMM or similar products
for crystallization (optimization). Haven't find any companies selling
these items. Any suggestions will be greatly appreciated.
Thank you.
Jobichen Chacko
Dear All,
Here comes the problem of blind reveiw, the authors are always at the
receving end to share all there data, results and now the full
cordinates to an unknown person, just trusting the journal editor. Why
don't the journals think about making the name of the reviewer also
public.
Eventhou
Dear All,
When running refmac with twin refinement option it continues
indefinitely at "trying gamma equal" step. With normal refinement
this problem is not there.
Any idea, how to rectify this?
Jobi
it is also running now.
Thanks for all who gave valuable suggestions.
Jobi
On Thu, Oct 6, 2011 at 11:26 PM, Jobichen Chacko wrote:
> Dear All,
> I even tried to remove the whole ccp4i directory and installed it
> fresh using the same tar file. But this problem is recurring again. I
Dear All,
I even tried to remove the whole ccp4i directory and installed it
fresh using the same tar file. But this problem is recurring again. It
seems some bug is already installed in the system which prevents the
execution of the program.
I searched in the CCP4 page and found some info. That pa
elete the CCP4-database directory and its contents.
Jobi
On Wed, Oct 5, 2011 at 9:59 AM, Jobichen Chacko wrote:
> Dear All,
> I am getting the following error message when trying to execute any program.
>
> Database Access Failure
>
> This instance of CCP4i no longer has
Dear All,
I am getting the following error message when trying to execute any program.
Database Access Failure
This instance of CCP4i no longer has control of the current database..
It is recommending to close the interface and restart, but it is
coming back with the same error even after r
Dear All,
Thank you for the replies to my post. I received more than 14 response. The
summary is given below.
The suggestions were
- add another purification step.
* Ni-column
* remove His-tag
* Ni-column
* gel filtration
if you have done that: add another step to test for better crystals
Dear All,
We got crystals for a 35 KDa protein with 323aa including His tag and
linker. It was originally crystallized in 0.1M BisTris Propane pH:6.5, 0.2M
Potassium thiocyanate and 20% PEG 3350. Later we managed to obtain crystals
with 0.1M BisTris pH:6.5, 0.2M Potassium thiocyanate and 20% PEG
It is really shocking news. He will remembered throughout the world for his
excellent tool, PYMOL. May his soul rest in peace.
Jobi
On Fri, Nov 6, 2009 at 9:10 AM, Lunin, Vladimir wrote:
> What a sad news! It’s a great loss for us.
>
> Still can’t believe it.
>
> Warren was very helpful and res
Congratulations to the eminent scientists.
Jobi
On Thu, Oct 8, 2009 at 12:52 AM, Eleanor Dodson wrote:
> A Leslie wrote:
>
>> BB members may be interested to know that the 2009 Nobel prize for
>> chemistry has been awarded to Venki Ramakrishnan, Tom Steitz and Ada Yonath
>> for their structural w
I also some times seen that proteins are not sticking that much with
vivaspin concentrrtors. These are transparent concentrators and it is easy
to collect the concentrated protein by giving an inverted spin for 1
minute. Please check the website for more details www.vivascience.com
Jobi
On Wed,
Hi Raja,
There is a program called MAPMAN, you can use this program to convert CNS map into CCP4 format and vice versa.
Jobi
From: CCP4 bulletin board on behalf of Raja DeySent: Fri 5/2/2008 7:57 AMTo: CCP4BB@JISCMAIL.AC.UKSubject: [ccp4bb]
Hi, Is it possible to display cns map in coot? I
Dear All,
I am refining a low resolution structure of 3 Angstrom. I refined the
structure to 25 R value and 29 R free. But some of the residues are
showing very low B factors (less than 5).Please advice me how to tackle
this problem. As mentioned in a previous post I used babinet scaling in
Refamc
Dear All,
Can you please inform me the programs available to find whether a crystal is
twinned and also the data reduction programs for twinned crystals.
Thanks in advance.
Jobi
From: CCP4 bulletin board on behalf of Ian Tickle
Sent: Sat 9/1/2007 1:46 AM
To: CCP4
.
Cross-seeding a complete screen with microcrystals.
Crystallization in sitting drops under oil.
Jobichen Chacko.
Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the
condition to improve the size of the crystal and reduce the number of
nucleation.
The crystals are coming from five to six conditions , all have PEG 3350 in
common.
Apart from PEG, t
25 matches
Mail list logo
