Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the 
condition to improve the size of the crystal and reduce the number of 
nucleation.
The crystals are coming from five to six conditions , all have PEG 3350 in 
common.
Apart from PEG, the condition has Lithium sulphate and Bis Tris in one 
condition and Ammonium suphate and HEPES in another condition, while the third 
condition is Sodium malonate, Bis Tris  and PEG. I am getting thousands of 
small crystals with some precipitation.
I tried macroseeding and additive screen, but the crystals are not growing.
Any suggestions are more than welcome.
Thank you.
Jobi
 
 

 

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