Re: [ccp4bb] Help crystalizing protein

I'll second limited proteolysis with chymotrypsin. Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent from Proton Mail Android Original Message On 10/01/2025 07:02, Jeroen Mesters wrote: > Hi, > > three things come to mind besides what has been mentioned already > > - m

Re: [ccp4bb] Issues with MacOS 15.2

Hi Jiazhi, I found that ccp4i2 worked after deleting my local Python3.9 library and before re-installing Xquartz. Thank you for bringing Xquartz to my attention; I had overlooked that it needed to be replaced, too. Best regards, Blaine From: Tang, Jiazhi Sent

Re: [ccp4bb] Help crystalizing protein

Hi Marco, In addition to what has already been suggested, you could also try co-crystallising with a peptide from the partner protein that was used for the existing structure. Specifically, use a peptide from and interacting surface, which might help with the crystal packing, as well as stabili

Re: [ccp4bb] Issues with MacOS 15.2

Dear all, Thanks all for your generous suggestions. It turns out install XQuarts and grants it full access perfectly solved the issue. Again, appreciate your kind suggestions! Regards, Jiazhi Sent from Outlook for iOS From: CCP4 bulletin

Re: [ccp4bb] Issues with MacOS 15.2

Hi Jiazhi, Thank you for the post. I, too, have a new m4 Mac with OS 15.2 Sequoia. I had installed ccp4-9 two days ago, but only checked that mtzdump ran in the terminal (it did). I had not checked ccp4i. I can confirm some trouble similar to yours. Some of my troubles were created by transferri

Re: [ccp4bb] Issues with MacOS 15.2

Dear Jiazhi Do you have XQatrz installed? Any error messages during installation? Another reason might be permissions: Go to System Settings > Privacy & Security and ensure that CCP4 and XQatrz have Full Disk Access Warm regards, Maria Fando CCP4 Core team UKRI Scie

Re: [ccp4bb] Issues with MacOS 15.2

Hi Jiazhi, Good luck with you new adventures! You may want to contact specifically the CCP4 core team and they should be able to help. They may not have seen you email on the ccp4BB as we were at the study weekend the past 3 days. You can contact me off list if you don't manage to get a conta

[ccp4bb] Fwd: 2-year postdoctoral position in AI based Protein design at the Spanish Research Council (CSIC), Madrid, Spain

Dear all, just a reminder about the position below. We will still be collecting CVs for another 2 weeks. Best regards, Daniel -- Forwarded message - From: Daniel Lietha Date: Fri, Dec 20, 2024 at 10:33 AM Subject: 2-year postdoctoral position in AI based Protein design at the Spa