[ccp4bb] Post-doc position - Columbia University

A Post-doctoral Research Associate position is available for structural studies on proteins involved in RNA processing and quality control, using cryo-EM and crystallography. We have extensive experience in these areas, and have generated many high-impact publications (for example Science, 367, 700

Re: [ccp4bb] Elemental Analysis

As a follow-up, we have a postdoc position open to further develop metal identification methods for crystals and in solution! The project: structural study of biological systems that are impacted by free radical activity and metalloproteins, where the modeled metal and/or coordination state are

Re: [ccp4bb] Elemental Analysis

Dear Andy, We can easily reach the Ca absorption edge on I23 (Diamond long wavelength beamline), so we can tell you if you have calcium in your crystal but also its location. Have a look at the beamline webpage: https://www.diamond.ac.uk/Instruments/Mx/I23.html Cheers, Kamel

Re: [ccp4bb] Elemental Analysis

Hi Andy, We were able to use EDX fluorescence scans at a beam line to detect calcium in crystals. https://pubmed.ncbi.nlm.nih.gov/29890074/ It isn't quantitative like micro-pixe but you can see it... Eddie Snell and Elspeth Garman and team have done some further work on micro-pixe, as well –

[ccp4bb] Elemental Analysis

Dear Andy Yes! I have regular runs of micro-PIXE. Happy to discuss with you off line. Best wishes Elspeth From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> On Behalf Of Andrew Lovering Sent: 07 January 2021 14:13 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Ele

Re: [ccp4bb] Elemental Analysis

Hi Andrew, We had considerable success with MicroPIXE collaborating with the Garman group in Oxford and the Surrey Ion Beam Center. There is a tiny amount of sample needed, it's an atomic technique so the sample can be from that old tube that hadn't been discarded yet, and it provides identific

Re: [ccp4bb] Elemental Analysis

Hello again, re: amount, yes, from memory, it was a few milligrams. We also used EDAX on dried samples IIRC. Cheers, Jon.C. Sent from ProtonMail mobile Original Message On 7 Jan 2021, 14:13, Andrew Lovering wrote: > Dear CCP4ers > > We have a protein with moderate resolution (

Re: [ccp4bb] Elemental Analysis

Hello, there is atomic-absorption. Breaks your heart to see the protein going into the flame, but we got good results for Se and transition metals with a superoxide dismutase. I doubt if there'll ever be one of those on a beam line ;-0 Cheers, Jon Cooper. Sent from ProtonMail mobile O

[ccp4bb] Elemental Analysis

Dear CCP4ers We have a protein with moderate resolution (mid 2s) with obvious metal ion density holding the oligomer together. We can make an educated guess that the ions are likely to be Mg/Ca and can look at co-ordination and bond lengths.but I have a more specific question - would it be

[ccp4bb] MX Tutorials satellite to ESRF User Meeting 2021

Dear colleagues, we would like to draw your attention to two Tutorials that are organized satellite to the virtual ESRF User Meeting 2021 that might be of your interest: *Remote data collection with EXI and MXCuBE3 *and* Getting the best out of MASSIF-1 and workflows at the ESRF* The registration