Re: [ccp4bb] Are there any proteins capable of crystallizing at a wide range of pH having the same space group?

As far as lysozyme is concerned, the space group obtained is mainly the result of an interplay between the pI of lysozyme on the one hand and the pH of the solute and type of salt (or organic small molecule) on the other hand (Hofmeister series). The pI is about 9.5 and one should not be surpri

Re: [ccp4bb] Are there any proteins capable of crystallizing at a wide range of pH having the same space group?

Following my last question I was searching for a redundant PDB and found this paper https://doi.org/10.1016/j.str.2007.07.012. I went to the methods section and found out that the link to the database of redundant protein structures is now gone (http://dper.burnham.org/). Anyone knows of a similar

Re: [ccp4bb] Are there any proteins capable of crystallizing at a wide range of pH having the same space group?

My recollection is that gamma-chymotrypsin crystals will persist in pHs all the way from 3 to 9. I don't know if the crystals will grow over that range. There are a fair number of phage T4 lysozymes variants in the PDB. I don't think this is considered "overpopulation" but a valuable contr

[ccp4bb] Are there any proteins capable of crystallizing at a wide range of pH having the same space group?

A quick glance at the entries of hen egg white lysozyme in the PDB show that it can be crystallized at different pH values, but the space group is not always the same. I still have to refine the analysis but I was wondering that maybe there are a few proteins that can crystallize at a wide range (m

Re: [ccp4bb] Optimisation of 2D very thin plates to thick pl

Adding chymotrypsin to your crystallisations in about 1:100 mass ratio with your protein can help. You can quite easily find some papers on this method. #yiv8007840479 -- filtered {panose-1:2 4 5 3 5 4 6 3 2 4;}#yiv8007840479 filtered {font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;}#yiv800

Re: [ccp4bb] Optimisation of 2D very thin plates to thick plates

Hi Will, We had a similar case recently. Microseeding gave us single crystals (originally clusters), but still very thin plates as yours. We then mutated a couple surface lysine residues to alanine and that led to thicker crystals. The thin crystals were indexed as C2221, but the thicker ones rev

[ccp4bb] XChem fragment screening - call for proposals - 2nd October 5pm

Dear all, This is a reminder that Diamond Light Source is accepting proposals for its XChem fragment screening facility, for the allocation period April to September 2020. The deadline is Wednesday 2nd October at 5pm (2 weeks from now). Details on how to apply are here - we're oversubscribed, so

Re: [ccp4bb] Optimisation of 2D very thin plates to thick plates

Microseeding for new conditions? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118815/ On Sep 19, 2019, at 17:30, William Richardson mailto:william.richard...@nottingham.ac.uk>> wrote: Dear colleagues, I would like some advice on crystal growth optimisation. I have been trying to crystallis

Re: [ccp4bb] challenges in structural biology

Giving the vagaries of alternate splicing, is there one discrete number of human genes out there to be determined? And what percentage of the encoded mass of protein is actually structured? Another more biochemical challenge for structural biology is figuring out how to deal with weak cooperativ

[ccp4bb] MoRDa update

Dear All MoRDa is a pipeline for molecular replacement structure solution. A new update to the package is now available. The structure solution program is improved and database is extended . Both update and installation instructions are available from Morda homepage

[ccp4bb] AW: [ccp4bb] challenges in structural biology

Dear John, the „100,000 human genes“ is a long-standing myth broad forward by the initiators of the U.S. human genome sequencing projects in 1990. This large number completely contradicted all genetics and mutation data since the 1940th, but the sequencing community (genome, cDNA, EST) didn’t re