Hi Will, We had a similar case recently. Microseeding gave us single crystals (originally clusters), but still very thin plates as yours. We then mutated a couple surface lysine residues to alanine and that led to thicker crystals. The thin crystals were indexed as C2221, but the thicker ones revealed that it was actually P21.
Hope this helps. Cheers, Quyen Quyen Hoang, PhD Associate Professor of Biochemistry and Molecular Biology Adjunct Associate Professor of Neurology Primary Investigator of the Stark Neuroscience Research Institute Indiana University School of Medicine 635 Barnhill Drive, MS0013C Indianapolis, IN, 46202 (317)274-4371 > On Sep 19, 2019, at 12:16 PM, Phoebe A. Rice <pr...@uchicago.edu> wrote: > > Is it a complex with DNA? If so, vary the DNA ends? > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > Phoebe A. Rice > Dept. of Biochem & Mol. Biol. and > Committee on Microbiology > https://voices.uchicago.edu/phoebericelab/ > <https://voices.uchicago.edu/phoebericelab/> > > > From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK > <mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of William Richardson > <william.richard...@nottingham.ac.uk > <mailto:william.richard...@nottingham.ac.uk>> > Reply-To: William Richardson <william.richard...@nottingham.ac.uk > <mailto:william.richard...@nottingham.ac.uk>> > Date: Thursday, September 19, 2019 at 10:41 AM > To: "CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>" > <CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>> > Subject: [ccp4bb] Optimisation of 2D very thin plates to thick plates > > Dear colleagues, > I would like some advice on crystal growth optimisation. I have been trying > to crystallise a DNA regulator. I got microcrystals in 1.3M Lithium Sulphate > and 50 mM Cacodylate pH = 6. Through a combination of condition refinement > and seeding I have attained crystals with a plate-like habit of dimension > 50micron, 50micron, <1micron. These have very weak (8Å) anisotropic > diffraction at DLS i24 but as they’re so wafer thin there has been no > improvement. Any suggestions to make them more 3D would be great. The space > group is C2221 > Any advice? > Thanks in advance > Regards, > > Will > > > <image001.png> > > This message and any attachment are intended solely for the addressee > and may contain confidential information. If you have received this > message in error, please contact the sender and delete the email and > attachment. > > Any views or opinions expressed by the author of this email do not > necessarily reflect the views of the University of Nottingham. Email > communications with the University of Nottingham may be monitored > where permitted by law. > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
