Microseeding for new conditions? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118815/
On Sep 19, 2019, at 17:30, William Richardson <william.richard...@nottingham.ac.uk<mailto:william.richard...@nottingham.ac.uk>> wrote: Dear colleagues, I would like some advice on crystal growth optimisation. I have been trying to crystallise a DNA regulator. I got microcrystals in 1.3M Lithium Sulphate and 50 mM Cacodylate pH = 6. Through a combination of condition refinement and seeding I have attained crystals with a plate-like habit of dimension 50micron, 50micron, <1micron. These have very weak (8Å) anisotropic diffraction at DLS i24 but as they’re so wafer thin there has been no improvement. Any suggestions to make them more 3D would be great. The space group is C2221 Any advice? Thanks in advance Regards, Will <ExamplesOfPlates.png> This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please contact the sender and delete the email and attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. Email communications with the University of Nottingham may be monitored where permitted by law. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
