Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] crystal contacts and biologically relevant contacts

Lazy body's solution - read the PDB into pisa, and output the "assembly" . This will a) move multiple molecule copies in an asymmetric unit to symmetry versions which make the best biological sense, and b) if necessary add extra symmetry mates to make up an assembly. Example Heamog

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Re: [ccp4bb] image

Try seeding at lower concentration. From: CCP4 bulletin board On Behalf Of zhangyi19950109 Sent: Friday, March 22, 2019 1:55 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] image Dear all, A single plus-stranded RNA virus RDRP monomer grows crystals, precipitants (PEG8000) with low concentration

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Re: [ccp4bb] crystal picture

Hi Yi Zhang As Sharan suggests microseeding may be the key to solving your problem. ‘Random’ microseeding or MMS, where seed stock is added to random screens may be helpful. It often gives new hits, crystals that diffract better and may allow you to control the number of crystals per drop (if you

Re: [ccp4bb] crystal picture

Dear Jun Did you tried seeding the old crystals. Just crush them and make different dilutions with same buffer. Hope, it will work out for you. Regards Sharan Fri, Mar 22, 2019, 6:55 AM 马俊 wrote: > Dear all, >A single positive stranded RNA virus RDRP grows crystals, monomers, > precipita

[ccp4bb] crystal picture

Dear all, A single positive stranded RNA virus RDRP grows crystals, monomers, precipitants (PEG8000) with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution. Precipitant concentration increased, many cr

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[ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] crystal contacts and biologically relevant contacts

Hi Alex, Kevin is right. To clarify things a little, you don’t have to use the symmetry mate selected by the molrep program. You can take the symmetry mate that makes the relevant biological interactions instead. No need to change the space group. You have to delete the molecule that does not m