Dear all,
refinement of my protein structure involves a C-terminal amide. Looking
through the monomer/link library files mon_lib_list.cif and
mon_lib_ind.cif I could not identify a modification or link suitable
for amide restraints. I know I could construct a proper .cif myself,
however I a
On Mon, Dec 8, 2014 at 7:24 PM, Keller, Jacob
wrote:
> FYI, sometimes native nucleotides can make it through protein
> purifications if binding is tight.
>
This is especially true for G-proteins, since tight binding to GDP is an
essential part of their function. I don't know what to expect fro
Hi All;
Could some body explain why I am getting the error message when running
MR-Rosetta.
Message is " Sorry cannot locate a binary starting with
mr_protocol.default' in the directory
/home/phenix/rosetta_2014.34.57213_bundle/main/source/bin
I just download it and locate it in the preferences,
This is where it’s customary to include a small image or two (or better, a link
thereto) which shows the density, and the masters here can tell you there best
guesses—seems to be a bit of a parlour game. Also include info on what is in
the crystallization condition and protein buffer if you dare
Hi all,
We crystallised a small GTPase expressed in E. Coli and found some densities in
GDP/GTP binding site. We didn’t add any GDP/GTP or GDP/GPD homologues during
protein expression, purification and crystallisation. The resolution is not
high, we couldn’t tell what it is. Is there any method
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